diff --git a/src/__tests__/InvertedSeparator.test.tsx b/src/__tests__/InvertedSeparator.test.tsx
new file mode 100644
index 000000000..4cdfdc08d
--- /dev/null
+++ b/src/__tests__/InvertedSeparator.test.tsx
@@ -0,0 +1,95 @@
+import React from "react";
+import { Text, View } from "react-native";
+import "@quilted/react-testing/matchers";
+import { render } from "@quilted/react-testing";
+
+import { FlashList } from "..";
+
+jest.mock("../recyclerview/utils/measureLayout", () => {
+  const originalModule = jest.requireActual(
+    "../recyclerview/utils/measureLayout"
+  );
+  return {
+    ...originalModule,
+    measureParentSize: jest.fn().mockImplementation(() => ({
+      width: 399,
+      height: 899,
+    })),
+    measureFirstChildLayout: jest.fn().mockImplementation(() => ({
+      x: 0,
+      y: 0,
+      width: 399,
+      height: 899,
+    })),
+    measureItemLayout: jest.fn().mockImplementation(() => ({
+      x: 0,
+      y: 0,
+      width: 100,
+      height: 100,
+    })),
+  };
+});
+
+const Separator = () => <View style={{ width: 10 }} />;
+
+/**
+ * Returns, for every rendered cell, whether the separator sits before or after
+ * the item inside the cell. Cells are keyed by their item index.
+ */
+const separatorPlacementByIndex = (result: ReturnType<typeof render>) => {
+  const placement = new Map<number, "before" | "after" | "none">();
+  for (const cell of result.findAllWhere(
+    (node: any) => node.type === View && typeof node.props.index === "number"
+  )) {
+    const index = (cell as any).props.index as number;
+    if (placement.has(index)) continue;
+    const childTypes = React.Children.toArray((cell as any).props.children).map(
+      (child: any) => child.type
+    );
+    const itemAt = childTypes.indexOf(Text);
+    const separatorAt = childTypes.indexOf(Separator);
+    placement.set(
+      index,
+      separatorAt === -1 ? "none" : separatorAt < itemAt ? "before" : "after"
+    );
+  }
+  return placement;
+};
+
+const renderList = (inverted: boolean) =>
+  render(
+    <FlashList
+      data={[0, 1, 2, 3]}
+      horizontal
+      inverted={inverted}
+      overrideProps={{ initialDrawBatchSize: 1 }}
+      drawDistance={0}
+      renderItem={({ item }) => <Text>{`item-${item}`}</Text>}
+      ItemSeparatorComponent={Separator}
+    />
+  );
+
+describe("ItemSeparatorComponent placement", () => {
+  it("renders the separator after the item in a regular list", () => {
+    expect([...separatorPlacementByIndex(renderList(false)).entries()]).toEqual(
+      [
+        [0, "after"],
+        [1, "after"],
+        [2, "after"],
+        [3, "none"],
+      ]
+    );
+  });
+
+  it("renders the separator before the item in an inverted list", () => {
+    // Both the scroller and each cell are flipped, so a trailing separator
+    // would visually land between item i and item i - 1, leaving the two
+    // items at the far end of the list flush against each other.
+    expect([...separatorPlacementByIndex(renderList(true)).entries()]).toEqual([
+      [0, "before"],
+      [1, "before"],
+      [2, "before"],
+      [3, "none"],
+    ]);
+  });
+});
diff --git a/src/recyclerview/ViewHolder.tsx b/src/recyclerview/ViewHolder.tsx
index 54b840301..1f468bea2 100644
--- a/src/recyclerview/ViewHolder.tsx
+++ b/src/recyclerview/ViewHolder.tsx
@@ -139,8 +139,13 @@ const ViewHolderInternal = <TItem,>(props: ViewHolderProps<TItem>) => {
       style={style}
       index={index}
     >
+      {/* In an inverted list the scroller and every cell are both flipped, so a
+          cell's content reads normally while its neighbours are mirrored: the
+          item at index + 1 ends up on the cell's leading side. Render the
+          separator before the item so it still lands between the two. */}
+      {inverted ? separator : null}
       {children}
-      {separator}
+      {inverted ? null : separator}
     </CompatContainer>
   );
 };