diff --git a/tests/baseline/layers_max_opacity_2d_view-chrome-1.png b/tests/baseline/layers_max_opacity_2d_view-chrome-1.png
index bd7541f27..d21362f57 100644
Binary files a/tests/baseline/layers_max_opacity_2d_view-chrome-1.png and b/tests/baseline/layers_max_opacity_2d_view-chrome-1.png differ
diff --git a/tests/fixtures/layering/mra-head-neck-3-slices.zip b/tests/fixtures/layering/mra-head-neck-3-slices.zip
new file mode 100644
index 000000000..48f86eb91
Binary files /dev/null and b/tests/fixtures/layering/mra-head-neck-3-slices.zip differ
diff --git a/tests/fixtures/layering/prostate-3-slices.zip b/tests/fixtures/layering/prostate-3-slices.zip
new file mode 100644
index 000000000..20b5a91ae
Binary files /dev/null and b/tests/fixtures/layering/prostate-3-slices.zip differ
diff --git a/tests/specs/layers-and-rendering.e2e.ts b/tests/specs/layers-and-rendering.e2e.ts
index 66ecd9d04..a67be5fd0 100644
--- a/tests/specs/layers-and-rendering.e2e.ts
+++ b/tests/specs/layers-and-rendering.e2e.ts
@@ -1,7 +1,32 @@
-import { DOWNLOAD_TIMEOUT } from '@/wdio.shared.conf';
+import * as fs from 'fs';
+import * as path from 'path';
+import { cleanuptotal } from 'wdio-cleanuptotal-service';
+import { DOWNLOAD_TIMEOUT, FIXTURES, TEMP_DIR } from '@/wdio.shared.conf';
 import { volViewPage } from '../pageobjects/volview.page';
-import { openUrls } from './utils';
-import { PROSTATEX_DATASET, MRA_HEAD_NECK_DATASET } from './configTestUtils';
+import { openUrls, openVolViewPage, writeManifestToFile } from './utils';
+import { PROSTATEX_DATASET } from './configTestUtils';
+
+const openLayeringFixtures = async () => {
+  const fixtureNames = ['prostate-3-slices.zip', 'mra-head-neck-3-slices.zip'];
+  await Promise.all(
+    fixtureNames.map(async (name) => {
+      const target = path.join(TEMP_DIR, name);
+      await fs.promises.copyFile(path.join(FIXTURES, 'layering', name), target);
+      cleanuptotal.addCleanup(async () => {
+        fs.unlinkSync(target);
+      });
+    })
+  );
+
+  const manifestName = `layering-fixtures-${Date.now()}.json`;
+  await writeManifestToFile(
+    {
+      resources: fixtureNames.map((name) => ({ url: `/tmp/${name}` })),
+    },
+    manifestName
+  );
+  await openVolViewPage(manifestName);
+};
 
 describe('Layers and Rendering', () => {
   it('should show 3D rendering controls regardless of active view', async () => {
@@ -33,7 +58,7 @@ describe('Layers and Rendering', () => {
   });
 
   it('should create overlay with 2 DICOM images', async () => {
-    await openUrls([PROSTATEX_DATASET, MRA_HEAD_NECK_DATASET]);
+    await openLayeringFixtures();
 
     await browser.waitUntil(
       async () => {
@@ -46,6 +71,9 @@ describe('Layers and Rendering', () => {
       }
     );
 
+    const mraCard = await $('.volume-card[html-title^="**TOF"]');
+    await mraCard.click();
+
     const inactiveMenuSelector =
       '.volume-card:not(.volume-card-active) button[data-testid="dataset-menu-button"]';
 
@@ -87,6 +115,23 @@ describe('Layers and Rendering', () => {
     );
     await addLayerButton.click();
 
+    await browser.waitUntil(
+      () =>
+        browser.execute(() => {
+          const app = (document.querySelector('#app') as any)?.__vue_app__;
+          const pinia = app?.config?.globalProperties?.$pinia;
+          const imageCache = pinia?._s?.get('image-cache');
+          const layerId = imageCache?.imageIds?.find((id: string) =>
+            id.includes('::')
+          );
+          return layerId && imageCache.imageStatus[layerId] === 'complete';
+        }),
+      {
+        timeout: DOWNLOAD_TIMEOUT,
+        timeoutMsg: 'Expected the layer image to finish loading',
+      }
+    );
+
     const renderTab = await volViewPage.renderingModuleTab;
     await renderTab.click();
 
@@ -103,36 +148,57 @@ describe('Layers and Rendering', () => {
 
     const layerOpacitySliders = await volViewPage.layerOpacitySliders;
     const firstSlider = layerOpacitySliders[0];
+    const sliderThumb = await firstSlider.$('[role="slider"]');
+    const inputElement = await firstSlider.$('input');
+    const views2D = await volViewPage.getViews2D();
+    const viewCount = await views2D.length;
+    if (!views2D || viewCount === 0) {
+      throw new Error('Could not find 2D views for screenshot');
+    }
+    const firstView2D = views2D[0];
+    const beforeOpacityChange = await firstView2D.takeScreenshot();
 
-    await firstSlider.click();
+    await browser.execute((element) => {
+      (element as HTMLElement).focus();
+    }, sliderThumb);
 
-    // Use keyboard to set to maximum (End key goes to max)
     await browser.keys('End');
 
     await browser.waitUntil(
       async () => {
-        const inputElement = await firstSlider.$('input');
         const value = await inputElement.getValue();
         return value === '1';
       },
       {
-        timeoutMsg: 'Expected slider value to be 1 (max opacity)',
+        timeoutMsg: 'Expected slider value to be 1',
         timeout: 5000,
       }
     );
 
-    const views2D = await volViewPage.getViews2D();
-    const viewCount = await views2D.length;
-    if (!views2D || viewCount === 0) {
-      throw new Error('Could not find 2D views for screenshot');
-    }
-    const firstView2D = views2D[0];
+    await browser.waitUntil(
+      async () => (await firstView2D.takeScreenshot()) !== beforeOpacityChange,
+      {
+        timeout: 5000,
+        timeoutMsg: 'Expected the 2D view to render the opacity change',
+      }
+    );
+
     const result = await browser.checkElement(
       firstView2D,
-      'layers_max_opacity_2d_view'
+      'layers_max_opacity_2d_view',
+      {
+        pixelmatch: { threshold: 0.2 },
+        // Compare where the resampled prostate layer intersects the MRA.
+        blockOut: [
+          { x: 0, y: 0, width: 371, height: 130 },
+          { x: 0, y: 240, width: 371, height: 92 },
+          { x: 0, y: 130, width: 20, height: 110 },
+          { x: 340, y: 130, width: 31, height: 110 },
+        ],
+      }
     );
 
-    const THRESHOLD = 5; // percent
+    const THRESHOLD = 1; // percent
     await expect(result).toBeLessThan(THRESHOLD);
   });
 });